These springs flow to the surface through 500–600 m of permafrost in an area of gypsum-anhydrite diapiric uplift, and are suggested to originate in a subsurface salt layer recharged by an ice-covered alpine lake or sub-glacial meltwater. The Gypsum Hill (GH) springs (79° 24′ 30″ N, 90° 43′ 05″ W) are a collection of approximately 40 springs and seeps located on the bank of Expedition River. The cold saline springs on Axel Heiberg Island (AHI), located in the High Arctic in Nunavut, Canada, are rare examples of non-volcanic perennial springs discharging in an area of continuous permafrost. We detected Mars-relevant metabolisms including hydrogenotrophic sulfate reduction, sulfur reduction, and sulfide oxidation, which indicate the potential for microbial life in analogous S-rich brines on past and present Mars. We identified a rare surficial chemolithoautotrophic, sulfur-cycling microbial community active in a unique anoxic, cold, hypersaline Arctic spring. Low abundance and transcription of photoautotrophs indicated sulfur-based chemolithoautotrophy drives primary productivity even during periods of constant illumination. Despite low oxygen availability, sulfide oxidation was primarily attributed to aerobic chemolithoautotrophic Halothiobacillaceae. highly transcribed non-coding RNAs associated with transcriptional regulation, showing potential evidence of putative metabolic flexibility in response to substrate availability. Highly abundant and active sulfur-reducing Desulfuromusa sp. and was identified in phyla not typically associated with sulfate reduction in novel lineages of Spirochaetota and Bacteroidota. Sulfate reduction was dominated by hydrogen-oxidizing chemolithoautotrophic Desulfovibrionaceae sp. Genome-resolved metagenomics and metatranscriptomics were utilized to describe an active microbial community containing novel metagenome-assembled genomes and dominated by sulfur-cycling Desulfobacterota and Gammaproteobacteria. It perennially discharges cold (~ 7 ☌), hypersaline (7–8% salinity), anoxic (~ 0.04 ppm O 2), and highly reducing (~ − 430 mV) brines rich in sulfate (2.2 g.L −1) and sulfide (9.5 ppm), making Gypsum Hill an analog to putative sulfate-rich briny habitats on extraterrestrial bodies such as Mars. This release can be used with CLC Genomics Server 5.5.X.Gypsum Hill Spring, located in Nunavut in the Canadian High Arctic, is a rare example of a cold saline spring arising through thick permafrost.This release is based on CLC Assembly Cell 4.2.1.Fixed a bug in the Assemble Sequences tool causing some data sets to produce inferior contigs.Fixed a read mapper error occurring under special circumstances when excluding regions of a reference when mapping reads.Fixed an error when importing BAM files, including problems regarding download of reference sequences.Fixed error in importing SOLiD XSQ files.Fixed crash of detailed mapping report tool with certain data sets.Fixed problem that caused a crash with extract consensus sequence tool with certain parameter configurations and with read mappings with no reads.Fixed various stability and performance problems of Maximum likelihood phylogeny.Improved stability of Probabilistic variant detection on huge data sets.Previously, they were treated as unique reads. In BAM files created by BWA, non-specific reads are now recognized as such during import.If you are using the tool with annotations spanning across the starting point of a circular reference, we recommend re-running the analysis. Fixed: The Target Regions Statistics tool did not handle annotations covering the starting point of circular reference sequences properly.Fixed: Opening a search view for searching sequences at NCBI would sometimes fail.Fixed various problems related to launching the Workbench through Java Webstart.Any existing settings will be copied to the new location automatically.
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